Fig. 3From: Epigallocatechin gallate inhibits sphere formation of neuroblastoma BE(2)-C cellsEffect of EGCG on sphere formation in BE(2)-C cells. Parental cells were seeded into a 96-well plate, cultured in sphere medium (SM) containing the indicated concentrations of EGCG for 14–17 days, and examined by bright-field microscopy. The number of spheres (>100 μM in diameter) was counted manually. Sphere formation was defined as the percentage of spheres in each sample in relation to that in a control sample (without EGCG). The data shown are means ± SD of three independent experimentsBack to article page