Fig. 2

Effect of epigallocatechin gallate (EGCG) on cell proliferation and apoptosis of BE(2)-C cells. a Parental cells and spheres were seeded into a 24-well plate and treated with the indicated concentrations of EGCG. After 48 h, the number of cells was counted manually. Cell proliferation was defined as the percentage of cells in each sample in relation to that in a control sample (without EGCG). The data shown are means ± SD of three independent experiments. b Parental cells and spheres were seeded into a 6-well plate, treated with the indicated concentrations of EGCG for 48 h, and stained with propidium iodide (PI). Apoptosis was defined as the percentage of PI-positive cells in each sample analyzed by flow cytometry. The data shown are means ± SD of three independent experiments