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Table 4 Effects of IR exposure on mRNA expression and rhythm parameter of clock genes

From: Circadian effects of ionizing radiation on reproductive function and clock genes expression in male mouse

Clock genes index

Groups

CT 3:00

CT 7:00

CT 11:00

CT 15:00

CT 19:00

CT 23:00

M

A

ω

Ï•

Clock

(Relative mRNA expression)

Control

1.00±0.01

1.56±0.17

0.90±0.01

0.25±0.00

0.44±0.01

0.82±0.06

0.83

0.55

15

84.34

IR

1.38±0.12*

1.73±0.12

0.62±0.04*

0.24±0.03

0.23±0.02*

0.60±0.06

0.80

0.77

15

79.77

Bmal1

(Relative mRNA expression)

Control

1.00±0.11

1.45±0.12

0.67±0.05

0.21±0.02

0.03±0.00

0.45±0.03

0.63

0.66

15

90.51

IR

0.29±0.02*

0.46±0.05*

0.23±0.02*

0.11±0.01*

0.09±0.01*

0.19±0.02*

0.23*

0.16*

15

90.76

ROR- a

(Relative mRNA expression)

Control

1.00±0.10

0.19±0.02

0.62±0.06

1.99±0.16

4.15±0.49

1.49±0.13

1.58

1.63

15

283.80

IR

1.23±0.12

0.28±0.02*

0.19±0.02*

0.95±0.10*

3.02±0.23*

1.67±0.18

1.22

1.22

15

309.53

ROR-β

(Relative mRNA expression)

Control

1.00±0.09

0.32±0.02

0.83±0.08

2.38±0.25

6.37±0.43

3.03±0.32

2.32

2.62

15

290.16

IR

2.07±0.21*

1.70±0.13*

0.12±0.01*

0.31±0.02*

1.01±0.07*

3.55±0.31

1.46*

1.50*

15

13.11*

ROR-γ

(Relative mRNA expression)

Control

1.00±0.88

0.38±0.04

0.20±0.02

1.96±0.13

3.49±0.32

2.10±0.22

1.52

1.54

15

295.12

IR

0.41±0.04*

0.01±0.00*

0.44±0.03*

1.11±0.09*

2.31±0.27*

0.79±0.05*

0.84*

0.95*

15

278.88

  1. Note. Date are means ± standard error for the mRNA expression of clock genes including Clock, Bmal1, Ror-a, Ror-β, and Ror-γ. Asterisks indicate a significant difference from control at p < .05 at different Control Time (CT) hours. The data obtained were then fitted by the least squares method for cosinor analysis, M is the median of the rhythm, A represents the amplitude of the rhythm, ω is the radial frequency (15 °/h) and φ is the peak phase of the rhythm. The experimental mice were subjected to whole-body irradiation of 3 Gy X-rays at different Control Time (CT) hours: CT 3:00, CT 7:00, CT 11:00, CT 15:00, CT 19:00, and CT 23:00, respectively. Control mice were handled in the same manner as those in radiation-exposed groups but without X-ray. IR, ionizing radiation
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