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Table 1 The sequences of primers used in real-time PCR

From: Circadian effects of ionizing radiation on reproductive function and clock genes expression in male mouse

Gene

 

Primer sequence 5′ to 3′

Clock

Forward:

CGGCGAGAACTTGGCATT

Reverse:

AGGAGTTGGGCTGTGATCA

Bmal1

Forward:

GCAGTGCCACTGACTACCAAGA

Reverse:

TCCTGGACATTGCATTGCAT

ROR-α

Forward:

CCACCTACTCCTGTCCTCGTCAG

Reverse:

CTTCTGCACCTCGGCGTACAAG

ROR-β

Forward:

TGAGAATGTGTCGTGCCTTCAACC

Reverse:

CAGAACAGCAGAGGAGAACAGAGC

ROR-γ

Forward:

GTCCAGACAGCCACTGCATTCC

Reverse:

TGCCGTAGAAGGTCCTCCAGTC

β-actin

Forward:

GCTCTTTTCCAGCCTTCCTTCTTG

Reverse:

GTGTTGCATAGAGGTCTTTACGG

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Environmental Health and Preventive Medicine

ISSN: 1347-4715

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