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Fig. 5 | Environmental Health and Preventive Medicine

Fig. 5

From: Effect of IL-15 addition on asbestos-induced suppression of human cytotoxic T lymphocyte induction

Fig. 5

Proliferating and non-proliferating CD8+ lymphocytes. PBMCs were collected in each of the two experimental groups: cultured with allogenic stimulation under CB exposure, and cultured with allogenic stimulation under exposure to CB and IL-15. The expression level of granzyme B in proliferating and non-proliferating CD8+ lymphocytes, represented by CFSE-negative and CFSE-positive, respectively, were analyzed by FCM. a In each experimental group, the percentage of CFSE-negative cells in CD8+ cells is shown as the ratio to the allogenic-stimulated control. Data are obtained from five independent experiments using PBMCs and are shown as the mean + SD. Significant differences are marked with asterisks (*p < 0.05, **p < 0.01). b The dot plots with the parameters of granzyme B and CFSE were used to define granzyme B+ cells in proliferating (Q1) and non-proliferating (Q2) populations. The gated population of the CD8+ subset was employed for the plots. c The granzyme B expression level was shown in proliferating and non-proliferating CD8+ cells. Data are obtained from five independent experiments using PBMCs. The data are shown with the same symbol for each experiment. The control group cultured with allogenic PBMCs without CB is represented by (+), the group cultured with allogenic PBMCs under exposure to CB is represented by (+)/CB, and the group cultured with allogenic PBMCs under CB exposure with IL-15 is represented by (+)/CB/IL-15

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