Fig. 4

The expression level of granzyme B in CD8⁺ lymphocytes. PBMCs were collected from the three experimental groups: cultured with allogenic stimulation, cultured with allogenic stimulation under CB exposure, and cultured with allogenic stimulation under exposure to CB in media supplemented with IL-15. The expression of granzyme B was analyzed by FCM. a Representative histograms showing intracellular granzyme B expression levels in CD8⁺ lymphocytes. Gray-colored histogram shows a non-stained control in each panel. b In each experimental group, the percentage of granzyme B-positive cells in CD8⁺ cells was shown as the ratio to the allogenic-stimulated control. Data are obtained from three independent experiments and are shown as the mean + SD. Significant differences are marked with asterisks (^*p < 0.05). No significant difference is indicated by N.S.. The control group cultured with allogenic PBMCs without CB is represented by (+), the group cultured with allogenic PBMCs under exposure to CB is represented by (+)/CB, and the group cultured with allogenic PBMCs under CB exposure with IL-15 is represented by (+)/CB/IL-15