Fig. 3
From: Effect of IL-15 addition on asbestos-induced suppression of human cytotoxic T lymphocyte induction
The percentage of CD45RA-, CD45RO-, and CD25-positive cells in CD8+ lymphocytes. PBMCs were harvested from the three groups, representing allogenic stimulation, CB exposed with allogenic stimulation, and CB exposed with allogenic stimulation and IL-15, and assayed for the percentage of CD45RA-, CD45RO-, and CD25-positive cells using FCM. a Representative histograms of cell surface CD45RA and CD45RO expressed on CD8+ lymphocytes. Representative dot plots of CD25 versus CD8 on PBMCs. A non-stained control (gray) is shown in each panel. b Cumulative data showing percentage of CD45RA-, CD45RO-, and CD25-positive cells in CD8+ lymphocytes. Data showing the ratio of each group to the allogenic-stimulated control was calculated and compared among the groups for the percentage of CD45RA- and CD45RO-positive cells in CD8+ cells. Data represent the mean + SD from three independent experiments using PBMCs. Significant differences are indicated by asterisks (*p < 0.05, **p < 0.01). (+), the culture with allogenic PBMCs without CB; (+)/CB, the culture with allogenic PBMCs and CB; (+)/CB/IL-15, the culture with allogenic PBMCs and both CB and IL-15