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Fig. 1 | Environmental Health and Preventive Medicine

Fig. 1

From: Effects of maternal exposure to arsenic on social behavior and related gene expression in F2 male mice

Fig. 1

Experimental design. Pregnant C3H/HeN mice (F0) (n = 19) were used in this study. The arsenite mice (F0; n = 12) were exposed to sodium arsenite (NaAsO2, 85 ppm (85 mg/L) in the drinking water) from gestational days (GD) 8 to 18. The control mice (F0; n = 7) were given (arsenic free) tap water. The F1 male and female pups were weaned at postnatal day 21. At the age of 10 weeks, a mating was done among the control F1 males and females (1:2) to generate the control F2 mice. Similarly, to generate the arsenite-F2 mice, the arsenite-F1 males and females were mated (1:2). The F2 male pups (n = 18 for the control group: n = 27 for arsenite group) were weaned at postnatal day 21. At the age of 41 weeks, the control male mice (n = 9) and arsenite male mice (n = 12) were used for social behavior test and molecular analyses. At the age of 74 weeks, the control male mice (n = 9) and arsenite male mice (n = 15) were used for social behavior test and molecular analyses

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