Pilea umbrosa ameliorate CCl4 induced hepatic injuries by regulating endoplasmic reticulum stress, pro-inflammatory and fibrosis genes in rat

Table 1 Primer sequences for real-time PCR

Gene	Primer sequences (5′-3′)		Product size (bp)
GRP78	F R	GCAGTCTCCAGCCTACTTG TCCTTCCCCTTGAGAACCTG	103
PDI	F R	AGAACTCCAGGCGGTGTCT GCCATGGAACTGATGAGAG	150
XBP-1 s	F R	CATGGATTCTGACGCTGTTG CTCTGGGGAAGGACATTTGA	110
XBP-1 t	F R	TGTCACCTCCCCAGAACATC ACAGGGTCCAACTTGTCCAG	103
XBP-1u	F R	TGAAGCGCTGCGGAGGACA AGCTGGAGTTTCTGGTTCT	114
TNF-ɑ	F R	GTCTGTGCCTCAGCCTCTTC GCCATGGAACTGATGAGAG	122
IL-6	F R	GCCTGCAGAGATTCAAGTCA GTATCAGTGGGGGTCAGCAG	140
MCP-1	F R	TGTTCACAGTTGCTGCCTGT CGACTCATTGGGATCATCT	141
TGF-β	F R	GCCTGCAGAGATTCAAGTCA GTATCAGTGGGGGTCAGCAG	109
Smad-3	F R	CACCTCCTGGCTACCTGAGT GTTATTGTGTGCTGGGGACA	118
Col-1α	F R	TGTTCAGCTTTGTGGACCTC GACCCTTAGGCCATTGTGT	114
Nrf-2	F R	TCCAGACAGACACCAGTGGA GAATGTCTCTGCCAAAAGC	122
GCLC	F R	GAGAACATCAGGCTCTTTGC AGATGCACCTCCTTCCTCTG	106
β-actin	F R	TCTACAATGAGCTGCGTGTG ACGTACATGGCTGGGGTGT	134

GRP-78 Glucose regulated protein 78, PDI Protein disulfide isomerase, XBP-1 s X-box binding protein-1 spliced, XBP-1 t X-box binding protein-1 total, XBP-1u X-box binding protein-1 unspliced, TNF-α Tumor necrosis factor-alpha, IL-6 Interleukin-6, MCP-1 Monocyte chemoattractant protein-1, TGF-β Transforming growth factor-beta, Smad-3 Mothers against decapentaplegic homolog- 3, Col-1α Collagen-1 alpha, Nrf-2 Nuclear factor erythroid 2–related factor 2, GCLC Glutamate-cysteine ligase catalytic subunit

ISSN: 1347-4715