Fig. 6
From: Blocking the PD-1/PD-L1 axis enhanced cisplatin chemotherapy in osteosarcoma in vitro and in vivo
Increase of PD-L1 expression level in K7M2 cells. K7M2 cells were incubated with anti-PD-1 antibody (1 μg/ml) and cisplatin (2 μg/ml) alone or jointly for 48 h. a Immunohistochemical assay was performed to detect the expression of PD-L1 in K7M2 cell (× 400 magnification); the white arrow represents positive PD-L1 staining. b The optical density value of PD-L1 was statistically analyzed. The experiment was repeated three times. Results are presented as the mean ± standard deviation. **P < 0.01 and *P < 0.05 vs control group; #P < 0.05 vs cisplatin group. PD-L1, programmed death-ligand 1; PD-1, programmed cell death protein