Fig. 5
From: Blocking the PD-1/PD-L1 axis enhanced cisplatin chemotherapy in osteosarcoma in vitro and in vivo
Anti-PD-1 antibody synergized with cisplatin to promote cell apoptosis. K7M2-LV or K7M2-NC cells were co-cultured with PD-1+ lymphocytes, followed by incubation with anti-PD-1 antibody (1 μg/ml) and cisplatin (2 μg/ml) alone or jointly for 48 h. a Apoptotic cells were detected by flow cytometry. b The apoptotic rate of K7M2 cells was statistically analyzed. The experiment was repeated three times, and results are presented as the mean ± standard deviation. **P < 0.01 vs. the K7M2-LV + PD-1+ group; ##P < 0.01 vs. the control group (K7M2-LV + PD-1+); %%P < 0.01 vs. the control group (K7M2-NC + PD-1+). PD-1, programmed cell death protein