Fig. 2
From: Blocking the PD-1/PD-L1 axis enhanced cisplatin chemotherapy in osteosarcoma in vitro and in vivo
Detection of lentivirus transfection efficiency in K7M2 cells. K7M2 cells were transfected with the lentivirus carried both of GFP or PD-L1 gene for 72 h. a Images of K7M2 cells were captured using a fluorescent microscope following transfection with lentivirus (× 100 magnification). b RT-qPCR assay was applied to detect the PD-L1 mRNA expression level. *P < 0.05. The experiment was repeated three times, and results are presented as the mean ± standard deviation. GFP, green fluorescent protein; PD-L1, programmed death-ligand 1