Table 1 Description of 4 SNPs within IL-6 gene and the primers designed
SNPs | Chromosome | Functional consequence | Restriction endonuclease | Alleles | Primer (5′→3′) |
|---|---|---|---|---|---|
rs1800795-174G/C | 7:22727026 | Intron variant, upstream variant 2KB | SfaNI | G/ C | F: 5′-TGACTTCAGCTTTACTCTTGT-3′ R: 5′-CTGATTGGAAACCTTATTAAG-3′ |
rs1800796-572C/G | 7:22726627 | Intron variant, nc transcript variant, upstream variant 2KB | BSrBI | C/ G | F: 5′-AGATTCCAAGGGTCACTTG-3′ R: 5′-AGAAGCAGAACCACTCTTC-3′ |
rs1554606 185C/A | 7:22729088 | Intron variant, upstream variant 2KB | EcoNI | C/ A | F: 5′-ACTCATACATCTGGCCGTTG-3′ R: 5′-CTGATGGAGTATGGTGGAGG-3′ |
rs2069849 | 7:22731537 | Downstream variant 500B, missense, synonymous codon | BsrDI | C/ T | F: 5′-CGTGCATGACTTCAGCTTTACT-3′ R: 5′-CAGTGACCAGATTAACAGGCTAGA-3′ |