Fig. 2
DEHP metabolite concentrations excreted in 24-h urine. Data indicates the mean ± standard deviation. Each letter represents grouping, and different letters indicate significant differences (p < 0.05), i.e., there were significant differences between the groups A and B, A and C, and B and C. White and black columns indicate urinary metabolites in 20-week-old male mice fed with control and 0.1% DEHP-containing food for 8 weeks, respectively. These metabolites were measured by gas chromatography-mass spectrometry in Kansai Technical Center for Occupational Medicine, Japan. The detailed methods were described elsewhere [12]. #The values of 2cx-MMHP contain a part of PA. WT, wild-type mice; KO, Pparα-null mice; HT, PPARα-humanizedTet-Off mice; +/+, homozygous; +/−, heterozygous; MEHP, mono(2-ethylhexyl)phthalate; 5oxo-MEHP, mono(2-ethyl-5-oxohexyl)phthalate; 2cx-MMHP, mono[2-(carboxymethyl)hexyl]phthalate; 5cx-MEPP, mono(2-ethyl-5-carboxypentyl)phthalate; 2EH, 2-ethyl-1-hexanol; PHA, phthalic anhydride; Cr, urinary creatinine. n = 5–7