Fig. 2

Vestibular hair cell loss in the saccule, utricle, and cupula of mice treated with IDPN. a–c After administration of IDPN, hair cells were stained with anti-MyosinVIIa antibody. Equivalent positions in the saccule (a), utricle (b), and cupula (c) from mice in the control group (left panels) and mice in the exposure group (right panels) are shown with the same scale (scale bar 20 μm in a). An asterisk shows an example of normal hair cells (a), and arrows indicate loss of hair cells (a–c, right panels). d, e Numbers of hair cells per 100 μm (mean ± SD) in the saccules (d) and utricles (e) from 3 mice in the control group (gray) and 3 mice in the IDPN group (white) are shown. f Numbers of hair cells in the cupula (means ± SD) from three mice in the control group (gray) and 3 mice in the IDPN group (white) are shown. Significant differences (*p < 0.05, **p < 0.01) between the two groups were analyzed by Welch’s t test