Fig. 5
Effect of glutamine combined with LY294002 or IGF-1 on SOD and GSH-Px activity and MDA content caused by MPP+ in PC12 cells. a PC12 cells were incubated with LY294002 (20 μM) or IGF-1 (100 ng/ml). The PI3K, Akt, and P-AKT proteins were examined by western blotting. b The relative intensity of proteins was shown as a bar graph. c PC12 cells were incubated with MPP+ (227 μM) for 48 h following pre-treatment with glutamine (64 μM) and/or LY294002 (20 μM) and/or IGF-1 (100 ng/ml) for 1 h. SOD and GSH-Px contents in each group. d MDA content in each group. Data were obtained from three independent experiments. The results were presented as the mean ± standard deviation. *p < 0.05 and **p < 0.01, compared with control group. #p < 0.05 and ##p < 0.01, compared with M0 group. %p < 0.05 and %%p < 0.01, compared with M0+G0 group. SOD, superoxide dismutase; GSH-Px, glutathione peroxidase; MDA, malondialdehyde