Fig. 2

Chronic noise exposure increases the expression of CRF, CRFR1, and CRFR2 in the hippocampus. Comparison of CRF, CRFR1, and CRFR2 mRNA expression levels in control and noise-exposed rats by quantitative real-time PCR at various time points following the cessation of noise exposure (a, d, g). Western blot analysis of hippocampal CRF, CRFR1, and CRFR2 expression under C (control) and N (chronic noise exposure) conditions (b, e, h). GAPDH was used as a loading control. Quantification of immunoreactive band density measured in panels b, e, and h was normalized against GAPDH (c, f, i). Data are presented as the percentage changes relative to control samples. Bars represent means ± SD *p < 0.05, compared with respective controls (n = 6 per group)