Fig. 2

Representative images of the in vitro mature biofilms on polystyrene at 20 °C (a) and 37 °C (b) and on stainless steel coupon at 20 °C (c) and 37 °C (d) of E. hirae ATCC 10541, and the effect of disinfectants LH IDROXI FAST (inserts on the left) and LH ENZYCLEAN SPRAY (inserts on the right) tested at the same conditions. Biofilms were cultured for 48 h, stained with live/dead reagents, and visualized with the optical microscope fluorescence. Sessile population in biofilms stained in red (propidium iodide) expresses a compromised membrane integrity (damaged), whereas green stained bacteria (SYTO 9) remained viable. Original magnification ×1000