Table 2 Oligonucleotide primer sequences and PCR conditions for real-time RT-PCR
From: Sasa veitchii extracts suppress acetaminophen-induced hepatotoxicity in mice
Gene (accession no.) | Primer sequences | PCR product length (bp) | |
|---|---|---|---|
Sequence (5′ to 3′) | |||
Cyp1a2 | Forward | GAC ACC TCA CTG AAT GGC TTC | 103 |
(NM_009993) | Reverse | ACA CAA AGG GGT CTT TCC ACT G | |
Cyp2e1 | Forward | CAT TCC TGT GTT CCA GGA GTA CAA G | 91 |
(NM_021282) | Reverse | GAT ACT TAG GGA AAA CCT CCG CAC | |
GAPDH | Forward | TGG TGA AGG TCG GTG TGA AC | 98 |
(NM_008084) | Reverse | GTC GTT GAT GGC AAC AAT CTC C | |