Fig. 4

SE pretreatment protects animals from acute APAP-induced hepatotoxicity, as assessed by H&E staining. Animals treated as in Fig. 1 were euthanized at 24 h post-intraperitoneal injection, and the livers were harvested at necropsy. Liver specimens were fixed and processed by standard methods, and sections were stained with H&E. These micrographs provide ×4 (a, b, c) and ×10 (d, e, f) magnified images of representative H&E-stained sections of the livers obtained from control (a, d), APAP (b, e), and SE + APAP (c, f) group animals. The image in b and e reveals severe necrosis and apoptosis in an APAP-exposed animal. In contrast, although necrosis is observed around the central vein in the SE + APAP group, the necrosis area is lower than that in the APAP-exposed group