Regulation of serine protease inhibitor Kazal type-5 (SPINK5) gene expression in the keratinocytes

Table 1 Primers to obtain SPINK5 promoter

For initial clone		Downstream primer	Promoter included
−1141	SacI-CTGGAATGAAACAATTTGGCTCTGG	−1	−1141/−1
−1	NheI-TGCTCAGCTGGTGCAGTATGACT
For deletion
−1076	SacI-GCTATTAGGGCAAAATAAGCTGGTA	pGL4	−1076/−1
−932	SacI-GAGACTAAATAATGGGATGATACAGG	pGL4	−932/−1
−861	SacI-CGGTAATATGGGTAATCATAGGCTA	pGL4	−861/−1
−676	SacI- GGATGAAGCAGTGTACAGAGAGG	pGL4	−676/−1
−555	SacI-AGTTCCTGCCTCCCTATATATTCA	pGL4	−555/−1
−437	SacI-CCCTGGATTTCTCTGGATGTGGA	pGL4	−437/−1
−318	SacI-GTCTACGAGGTAGTATATCTGGTAT	pGL4	−318/−1
−169	SacI-ATTCTCTGTTCAAACCTGACACCC	pGL4	−169/−1
pGL4	Sac I-AGGCCAGAGAAATGTTCTGGCACC
For fragmentation
−555c	TGAATATATAGGGAGGCAGGAACT	−677	−676/−532
−169c	GGGTGTCAGGTTTGAACAGAGAAT	−318	−318/−146

Numbers indicate the location of 5′ terminal base counted from the transcription initiation site. Primers −555c and −169c were complementary to −555, and −169, respectively, and hence the numbers were the locations of 3′ terminal base

ISSN: 1347-4715