Simultaneous changes in high-fat and high-cholesterol diet-induced steatohepatitis and severe fibrosis and those underlying molecular mechanisms in novel SHRSP5/Dmcr rat

Table 1 Effects of high-fat and cholesterol-containing diet on body and liver weight and on biochemical characteristics in plasma and liver at each treatment period

Diet treatment group:	SP-diet group			HFC-diet group
Treatment period:	2 weeks	8 weeks	16 weeks	2 weeks	8 weeks	16 weeks
Body and liver weight
Body weight (g)	224 ± 17	256 ± 13	271 ± 20	200 ± 25	237 ± 15	260 ± 39
Liver weight (g)	6.4 ± 0.5	6.6 ± 0.3	7.0 ± 0.4	8.6 ± 0.7**	23.1 ± 2.4**	31.1 ± 2.6**
Liver weight/body weight ratio	0.03 ± 0.001	0.03 ± 0.001	0.03 ± 0.002	0.04 ± 0.005**	0.10 ± 0.008**	0.12 ± 0.009**
Plasma measurements
Triglyceride (mg/dl)	53.9 ± 20.2	41.4 ± 7.1	72.6 ± 18.0	71.4 ± 22.9	59.1 ± 11.7*	366.2 ± 276.6*
Total cholesterol (mg/dl)	29.7 ± 7.4	45.1 ± 7.6	47.1 ± 12.6	135.3 ± 57.0**	112.6 ± 20.6**	213.0 ± 116.8*
ALT (IU/l)	16.7 ± 1.4	15.5 ± 1.4	23.3 ± 4.5	28.7 ± 3.8*	35.5 ± 3.5**	103.4 ± 59.1*
AST (IU/l)	58.2 ± 12.3	101.6 ± 11.6	103.2 ± 25.9	68.8 ± 10.4	138.5 ± 35.6	245.5 ± 117.4*
Total adiponectin (μg/ml)	5.4 ± 1.0	5.8 ± 0.6	6.1 ± 0.9	7.1 ± 0.8*	4.7 ± 0.4*	5.7 ± 1.1
TNF-α (pg/ml)	1.7 ± 0.3	2.4 ± 0.6	2.5 ± 1.6	4.7 ± 1.9*	19.1 ± 7.8*	13.7 ± 1.0*
Liver measurements:
Triglyceride (mg/g liver)	13.4 ± 3.9	23.8 ± 18.2	17.2 ± 3.9	47.8 ± 5.7**	31.8 ± 3.5	12.5 ± 4.3
Total cholesterol (mg/g liver)	2.3 ± 0.5	1.7 ± 0.5	1.6 ± 0.1	100.2 ± 6.6**	170.0 ± 12.9**	170.8 ± 24.6*

Data are presented as the mean value ± standard deviation (SD) for each group
SP-diet, Stroke-prone control chow; HFC-diet, high-fat and high-cholesterol-containing diet; ALT alanine aminotransferase; AST, alanine aminotransferase; TNF-α, tumor necrosis factor-α
*p < 0.05, **p < 0.01; compared with the SP-diet group within each diet treatment period

ISSN: 1347-4715