Fig. 2

H&E staining of liver in rats fed SP and HFC diets [original magnifications were ×100 (a–f) and ×400 (g–l)]. Liver from rats fed SP diet for 2 (a, g), 8 (b, h) and 14 weeks (c, i); liver from rats fed HFC diet for 2 (d, j), 8 (e, k), and 14 weeks (f, l). Lipid deposition (microvesicular steatosis predominantly) and focal or spotty necrosis with lymphocyte infiltrations (arrows) were observed in the liver of rats fed the HFC diet for 2 weeks (d, j). Extensive lipid deposition (macrovesicular steatosis predominantly) and multilobular necrosis in the liver of rats fed the HFC diet for 8 weeks (e, k). In addition to these findings, fibrosis was suspected in the liver of rats fed the HFC diet for 14 weeks (f, l). Mallory-Denk bodies (yellow arrows) were also observed in ballooned hepatocytes from rats fed the HFC diet for 8 and 14 weeks (k, l)