Fig. 1
From: Age-related effects of fasting on ketone body production during lipolysis in rats
Experimental protocol. Pre-feedings were carried out for 4 days. After the pre-feedings, rats in three age groups were divided into fasting (FAS) and control (CON) groups. Beginning on the next day, the 3-, 8-, and 32-week-old rats in the FAS group were deprived of food for 2, 5, and 12 days, respectively. During the experimental periods, body weights were measured. Plasma free fatty acid (FFA), total ketone body, β-hydroxybutyrate, and insulin concentrations were assayed. On the final days of fasting, the perirenal and periepididymal white adipose tissues were isolated and weighed (downward arrows)