Fig. 1

Stat5 activates the PPARγ2 promoter. a The sequence of murine and human PPARγ2 gene promoter was analyzed, and a potential Stat5 binding site in the promoters is indicated by the closed box (upper panel). Also is indicated a consensus Stat5 binding site. Schematic representation of the reporter gene constructs containing a wild-type Stat5 binding site (wild type), mutated Stat5 binding site (mutated nucleotides are underlined) or no Stat5 binding site (deleted) (lower panel). b Stat5 transactivates PPARγ2 promoter via Stat5-responsive element. Relative luciferase activity (firefly luciferase activity/renilla luciferase activity) was determined with the reporter constructs containing a Stat5 binding site, a mutated Stat5 binding site or no Stat5 binding site. After serum starvation, cells were left unstimulated or stimulated with FBS for 6 h. Results are expressed as fold induction and represent the mean ± standard deviation of three independent experiments. c Constitutively active Stat5A and Stat5B activate the PPARγ2 promoter in an FBS-independent manner. 3T3-L1 cells were transfected with the PPARγ2 reporter construct containing a Stat5 binding site together with wild-type or constitutively active Stat5 complementary DNA (cDNA) (Stat5A* or Stat5B*). After starvation, cells were left unstimulated or stimulated with 10% FBS for 6 h. Results are from three independent experiments performed in triplicates