Table 1 Evaluation of cross-reactivity with nicotine metabolites
From: Enzyme-linked immunosorbent assay of nicotine metabolites
Major nicotine metabolites | Molecular weight | ED50 (ng/mL) | ED50 (M) | Cross-reactive ratioa (%) |
|---|---|---|---|---|
Cotinine | 176.22 | 5.2 | 2.95 × 10−8 | 100.0 |
Nicotine | 162.23 | 2140.8 | 1.32 × 10−5 | 0.2 |
Nicotine glucuronide | 338.36 | 37919.8 | 1.12 × 10−4 | 0.0 |
Cotinine glucuronide | 352.34 | 3764.2 | 1.07 × 10−5 | 0.3 |
3′-Hydroxycotinine | 192.21 | 5.6 | 2.91 × 10−8 | 101.3 |
3′-Hydroxycotinineglucuronide | 368.34 | 4.9 | 1.33 × 10−8 | 221.8 |