Table 1 List of the selected genes used to upgrade the mouse Liver Stress Array ver. 1.0
Gene | Description | Full name | Accession no. | Forward primer 5→3 | Reverse primer 5→3 | Size (bp) |
|---|---|---|---|---|---|---|
Gsta2 | CAR-responsive | Glutathione S-transferase, alpha 2 | NM_008182 | GCAAAAGACAGGACCAAAA | GCACAATAGCCAGAATCAAC | 384 |
SLC21A6 | CAR-responsive | Liver-specific transporter-1/organic anion transporter | AF250912 | TTTTTCACTGCACTGGGAAG | TACAGGTTCTGGGTTTCCTT | 387 |
CAR | PXR-responsive | Constitutive androstane receptor | NM_009803 | CATCTCCAGGAGCCTGAGTA | CAGCATTTCATTGCCACT | 410 |
MRP2 | CAR, PXR-responsive | Multidrug resistance protein | NM_013806 | GATTGTTGAATACGGCAGTC | GGGGGTTTTAGGGATCA | 413 |
Sod1 | PPARalpha-responsive | Superoxide dismutase 1, soluble | NM_011434 | ACAGGATTAACTGAAGGCCAGC | ATGGTTTGAGGGTAGCAGATGAG | 430 |
Sucla1 | PPARalpha-responsive | Succinyl-CoA synthetase | NM_019879 | AGTCTTCCTGAATGATCCAGCC | TCCCACATACTGAGAGACCAACC | 450 |
Casp3 | Apoptosis | Caspase 3, apoptosis related cysteine protease | NM_009810 | GTCTTTGTACGCTACCACTGCCT | ATGGTGGTCCAACTCTTCACTTC | 418 |
Bax | Apoptosis | Bcl2-associated X protein | NM_007527 | TGACATGTTTGCTGATGGCA | TCCCACCCCTCCCAATAATT | 425 |
Cryz | AhR-responsive | Crystallin, zeta/quinone reductase | NM_009968 | AGTGATGCACACTACCACACACAG | GACCATACTGAAGACCACAGACCC | 470 |
PPARalpha | Nuclear receptor | Peroxisome proliferators-activated receptor alpha | NM_011144 | ACGAGGGTGTGATTTCATCCA | CAGGAACCAAGCCCCTCC | 417 |
Cpt2 | PPARalpha-responsive | Carnitine palmitoyltransferase 2 | NM_009949 | CAGTGAGCCTCGGTGGCTT | TCCAACCCGATCTCCTTAAGAA | 405 |
CEBPdelta | Transcription factor | CCAAT/enhancer binding protein, delta | NM_007679 | GCCTCTACTTTCCTCAATCT | GCACTGTCACCCATACAAT | 437 |
CyE1 | Cell cycle | Cyclin E1 | NM_007633 | GCAGATGCTGTGCTCTATGGAG | CATGAAGGCAGGACATGCTG | 401 |
Por | PXR-responsive | NADPH-cytochrome P450 oxidoreductase | NM_008898 | GCCCACAAGGTCTATGTTCA | CTAGGCTGCCTGGTACAATG | 422 |
Bak | Apoptosis | BCL2-antagonist/killer 1 | NM_007523 | TCTGAACACATCCATCAGGCTC | CCCTATGTTGTGCAAGACAGAGA | 388 |
Fah | Metabolizing enzyme | Fumarylacetoacetate hydrolase | NM010176 | AGAAGGAATGAGCCAGGCG | ATAAGGCAAGGGTGTGTCTTGTG | 403 |
AhR repressor | AhR-responsive | Aryl-hydrocarbon receptor repressor | NM_009644 | AGTGGCATGAATCCCAGCA | GAATGAGCAAAAGTGCAAGTTGG | 456 |
Ddit3 | Stress-responsive | DNA-damage inducible transcript 3/CHOP10/gadd153 | NM_007837 | GCAAGGAAGAACTAGGAAACGGA | CAATGTACCGTCTATGTGCAAGC | 394 |
Jun | Transcription factor | Jun oncogene | BC021888 | TGCCCCAACAGATCCCG | GCCCTGACAGTCTGTTCTCAAA | 403 |
m36B | Housekeeping gene | Acidic ribosomal phosphoprotein P0 (Arbp) | NM_007475 | CAACGGCAGCATTTATAA | CCCATTGATGATGGAGTGTGG | 171 |
Nrf2 | Transcription factor | Nuclear factor, erythroid-derived 2, like 2 | NM_010902 | GAAAAGGGAGAAAACGACAG | CCCAAATGGTGCCTAAGA | 429 |
Ccng2 | Cell cycle | CyclinG2 | NM_007635 | GGTGGTGTATTGTCCGATA | CATCACCACACAGAATTGCT | 438 |
MT1 | Stress-responsive | Metallothionein 1 | BC027262 | ACGACTTCAACGTCCTGAGTACCT | CACGTACTCGGTAGAAAACGGG | 382 |
MT2 | Stress-responsive | Metallothionein 2 | NM_008630 | AAACCGATCTCTCGTCGATCTTC | CAAGTCAACGGCTTTTATTGTCAG | 384 |
Gadd45a | DNA-damage-inducible | Growth arrest and DNA-damage-inducible 45 alpha | NM_007836 | CGGGAAAGTCGCTAC | AAAAATACCCAAACTATTGC | 421 |
Bad | Apoptosis | Bcl-associated death promoter | NM_007522 | CTGGACGCGCATTATCCAGT | GACCTCGGGTCCCCAGTTAT | 420 |
p21 | Cell cycle | Cyclin-dependent kinase inhibitor 1A | NM_007669 | CTTGGTGGTGAGACAGGCCT | TTATTGAGCACCAGCTTTGGG | 451 |
MRP1 | CAR, PXR-responsive | Multidrug resistance protein | AF022908 | GGAGAAAAGGTGGGTATTGT | GCCTCGTCCAACACTAGAAT | 410 |
CD36 | PPARalpha-responsive | CD36 antigen | NM_007643 | ACTTCAATTTCTGTAGACCC | CAGCATGGAACATGACG | 461 |
Lfas | PPARalpha-responsive | Long chain fatty acyl CoA synthetase | U15977 | GATGGCTGGTTACACACGGG | TCTTCGCCTTCAGTGTTGGA | 455 |
PXR | PXR-responsive | Pregnane X receptor | AF031814 | GTCGGCCATATCCTGCTC | GCGGGATTTGGAAGTTTATT | 397 |
Rad23b | DNA-damage-inducible | RAD23b homolog | NM_009011 | CCCATTCTTCGTGTTCA | TAAAGGCAGCAGTTTGACTC | 426 |
STA2 | CAR, PXR-responsive | Hydroxysteroid sulfotransferase (mSta2) | L27121 | ATGAAGACATGAAAAAGGAT | TTTCCACAACAGGTTTTATT | 413 |
Tnfrsf6 | Apoptosis | Tumor necrosis factor receptor superfamily, member6 | NM_007987 | ACCTTGGAAAATCAACCC | GTCTTCAATTAACTGCGAGT | 433 |
HSP60 | Stress-responsive | Heat shock 60-kDa protein | X53584 | GCGGCATGTTCTAACTCCTA | TCCACAGAAAGGCTGTCTTC | 413 |
MnSOD | Anti-oxidant enzymes | Manganese superoxide dismutase 2 | X04972 | GAGTTGCTGGAGGCTATCAA | CCAGTCATAGTGCTGCAATG | 423 |
OATP2 | PXR-responsive | Organic anion transporter 2 | AY007379 | CTCCTGCAGGCTGTATGACT | GCATACCTTTGGAAGCAGAA | 391 |
OSP | Stress-responsive | Oxidative stress-induced protein | U40930 | GGAATGTGTGATGCCTTTTC | CCATTAGAAAGGGGGACAGT | 435 |
p53 | Stress-responsive | Cellular tumor antigen p53 | X01237, K01700 | CTCCAGCTACCTGAAGACCA | GGTGGATAAATGCAGACAGG | 463 |
PCNA | Stress-responsive | Proliferating cell nuclear antigen | X53068 | TTTTTCACAAAAGCCACTCC | TGCATTTAGCGTCAAGATCA | 446 |
Rad50 | Stress-responsive | DNA damage repair and recombination protein 50 homolog | U66887 | AAGCCATCGGTGATCATTTA | GAGGTGACACCCAACAAATC | 469 |
Rad51 | Stress-responsive | DNA damage repair and recombination protein 51 homolog | D13473 | TCACAGGCTCATGTTGTGAC | TGCAGGGATCGTAGTCTAGC | 415 |
Tst | Metabolizing enzyme | Thiosulfate sulfurtransferase | S80191 | ACCGGAGCCGGATATAGTAG | AACCTACAGCAGGTCACACC | 415 |
Vim | Stress-responsive | Vimentin | X51438 | ATTGAGATCGCCACCTACAG | TCTTTTGGGGTGTCAGTTGT | 402 |