Development of a new disinfectant with very strong anti-influenza viral activity: a preliminary report

Table 2 Cell toxicity of the different disinfectants tested

Test reagent	Final concentration (% of the working solution)^a	Percentage inhibition of cell growth^b
Test reagent	Final concentration (% of the working solution)^a	CV-1	Jurkat
New disinfectant	10.0	60.2 ± 5.9	48.4 ± 4.2
	1.0	28.4 ± 3.8	22.9 ± 10.5
	0.1	18.2 ± 3.3	4.6 ± 3.0
	0.01	13.1 ± 9.1	0
	0.001	5.3 ± 2.3	1.6 ± 1.5
	0.0001	0	0
Chrolhexidine gluconate (positive control)	100	100	100
	10	99.3 ± 0.9	100
	1.0	82.0 ± 1.2	87.0 ± 14.6
	0.1	63.2 ± 5.4	31.0 ± 18.6
	0.01	19.9 ± 7.5	15.6 ± 6.6
	0.001	0.6 ± 0.8	8.6 ± 7.6

^aThe new disinfectant is meant to be used undiluted, and thus the working concentration is 100%. At the lowest test dilution, the new disinfectant was added at 10% (v/v) strength to the culture media. The recommended working dilution for chrolhexidine gluconate for disinfection of the skin is 0.1–0.5%; therefore, for the highest test concentration, a Hibitane concentrate containing 5% (w/v) chrolhexidine gluconate was added at 1/50 (v/v) to the culture media
^bData are presented as the mean ± standard error of the mean (SEM), calculated from the results of three repeated experiments

ISSN: 1347-4715