Fig. 4

The combination of isoliquiritigenin and TRAIL up-regulates the TRAIL receptor DR5. a HT29 cells were treated with the indicated concentrations of isoliquiritigenin for 24 h. Western blotting was performed with anti-DR5, DR4, and DcR2 antibodies. β-Actin was used as a loading control. As a positive control, a DR5 protein pattern after a tunicamycin treatment is shown [34]. b HT29 cells were treated as shown in a. Western blotting was performed with anti-Bcl-2, Bcl-xL, and BAX antibodies. β-Actin was used as a loading control. CT, treated with solvent DMSO. Arrows, DR5 protein bands; asterisks, non-specific bands. Band intensity is analyzed by ImageJ software and shown as bar graphs