Fig. 4

Identification of Itpr3 as a target of histone deacetylase (HDAC) in cardiomyocytes. a One of the DCS clones (H9C2T-2_D09; red rectangle) was mapped to chromosome 20p12, spanning intron 21 and exon 22 of Itpr3. Exons are denoted by black boxes, the arrow indicates the direction of transcription, and blue triangles depict distance markers separated by 50 kbp. b Chromatin immunoprecipitates were prepared from H9C2 cells treated (+) or not (−) with 300 nM inhibitor trichostatin A (TSA) for 24 h. The amount of DNA corresponding to the H9C2T-2_D09 sequence in each chromatin immunoprecipitation (ChIP) product relative to that in the corresponding original sample before immunoprecipitation (PreIP) was then determined by real-time PCR. c The amount of Itpr3 mRNA relative to that of Gapdh mRNA in H9C2 cells treated or not with TSA was determined by quantitative reverse transcription (RT)-PCR. All data are means ± SD of triplicates from representative experiments that were performed at least twice. P values for the indicated comparisons were determined by Student’s t test. Reproduced from Kaneda et al. [18]