Fig. 3

The differential chromatin scanning (DCS) method. DNA fragments bound to acetylated (Ac) histones are purified by immunoprecipitation (IP) and subjected to TAG adaptor ligation (green bars) and PCR amplification. The tester DNA is then digested with XmaI, ligated to the first subtraction adaptor (red bars), and annealed with an excess amount of the driver DNA. Given that only the tester-specific fragments self-anneal, PCR with the first subtraction primer selectively amplifies these fragments. The products are subjected to a second round of subtraction PCR with the second subtraction adaptor and primer to ensure the fidelity of the subtraction. Reproduced from Kaneda et al. [17]