Skip to main content
Download PDF
  • Original Article
  • Published:

Scavenging effects of methanolic extracts of broad beans on free-radical species

Environmental Health and Preventive Medicine volume 3, pages 6–11 (1998)Cite this article

An Erratum to this article was published on 01 July 1998

Abstract

This report describes the antioxidant characteristics of methanolic extracts from broad beans (Vicia fava). The methanolic extracts of broad beans (MEBB) exhibited a marked scavenging effect on superoxide. MEBB also exerted scavenging activities on hydrogen peroxide and 1, 1-diphenyl-2-picrylhydrazyl radical. The radical scavenging activity of MEBB was highest when the scavenging effect of MEBB on Superoxide (IC50 = 0.15 mg/ml) was examined. These results suggest that MEBB have effective activities both as a radical scavenger and as a hydrogen donor. The chelating activity of MEBB (0.70 mg/ml) on Fe2+ and Cu2+ was 31.2% and 28.5%, respectively. The antioxidant effect of MEBB on lipid peroxidation might be attributed to their properties of scavenging free-radical species and their chelating activity on metal ions. The antioxidant activity of MEBB against tert-butyl hydroperoxide (BHP)-induced oxidative stress in WI-38 cells was assessed. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase, and glutathione peroxidase (GSH-Px) were measured as indices of oxidative stress. WI-38 cells incubated with 0.1 mM BHP for 2 hr exhibited the increase of SOD, catalase and GSH-Px activities over the control. When the cells incubated in MEBB (45–450 μg/ml) for 18 hr were subjected to a BHP challenge test, SOD activity returned to its control value or lower at all levels tested. When catalase activity was determined, a similar trend occurred except in the cells incubated in 112.5 μ g/ml MEBB. These results imply that MEBB inhibit oxidative stress in WI-38 cells.

References

  1. Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci USA 1993;90: 7915–22.

    Article  PubMed  CAS  Google Scholar 

  2. Sohal RS, Allen RG. Relationship between oxygen metabolism, aging and development. Adv Free Rad Biol Med 1986;2: 117–60.

    Article  CAS  Google Scholar 

  3. Halliwell B, Gutteridge JMC, Cross CE. Free radicals, antioxidants and human disease: where are we now? Lab Clin Med 1992;119: 598- 620.

    CAS  Google Scholar 

  4. Klebanoff SJ. Oxygen Metabolism. In: Gallin JI, Goldstein IM, Snyderman R, editors. Inflammation: Basic Principles and Clinical Correlates. New York: Raven Press, 1992: 541–88.

    Google Scholar 

  5. Augusto O, Gatti RM, Radu R. Spin-trapping studies of peroxynitrite decomposition and of 3-morpholinosydnonimeineN-ethylcarbamide autooxidation: direct evidence for metal-independent formation of free radical intermediates. Arch Biochem Biophys 1994;310: 118–25.

    Article  PubMed  CAS  Google Scholar 

  6. Wang P, Zwier JL. Measurement of nitric oxide and peroxynitrite generation in the postischemic heart: evidence for peroxynitrite-mediated reperfusion injury. J Biol Chem 1996;271: 29223–30.

    Article  PubMed  CAS  Google Scholar 

  7. Herrmann K. Flavonols and flavones in food plants: a review. J Fd Technol 1976;11: 433–48

    CAS  Google Scholar 

  8. Kim SJ, Han D, Park MH, Rhee JS. Screening for superoxide dismutase-like compounds and its activators in extracts of fruits and vegetables. Biosci Biotech Biochem 1994;58: 2263–5.

    Article  CAS  Google Scholar 

  9. Lawlor SM, O’Brien NM. Astaxanthin: antioxidant effects in chicken embryo fibroblasts. Nutr Res 1995;15: 1695–704.

    Article  CAS  Google Scholar 

  10. Swain T, Hills WE. The phenolic constituents of Prunus Domestica L: The quantitative analysis of phenolic constituents. J Sci Food Agric 1959;10: 63–81.

    Article  CAS  Google Scholar 

  11. McCord JM, Fridovich I. Superoxide dismutase. An enzymic function for erythro cuprein (hemo cuprein). J Biol Chem 1969;244: 6049–55.

    PubMed  CAS  Google Scholar 

  12. Yen G-C, Duh P-D. Scavenging effect of methanolic extracts of peanut hulls on free-radical and active-oxygen species. J Agric Food Chem 1994;42: 629–32.

    Article  CAS  Google Scholar 

  13. Ruch KJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechin isolated from Chinese green tea. Carcinogenesis 1989;10: 1003–8.

    Article  PubMed  CAS  Google Scholar 

  14. Beers RF, Sizer IW. A spectrofluorometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 1952;195: 133–40.

    PubMed  CAS  Google Scholar 

  15. Blois MS. Antioxidant determination by the use of a stable free radical. Nature 1958;4617: 1198–200.

    Google Scholar 

  16. Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 1992;40:945–8.

    Article  CAS  Google Scholar 

  17. Yoshino K, Hara Y, Sano M, Tomita I. Antioxidative effects of black tea theaflavins and thearubigin on lipid peroxidation of rat liver homogenates induced bytert-butyl hydroperoxide. Biol Pharm Bull 1994;17: 146–9.

    PubMed  CAS  Google Scholar 

  18. Okada Y, Kimura H, Aoyama T. Neurotropin increasesin vitro life span of human fibroblasts. Mech Ageing Dev 1986;35: 133–43.

    Article  PubMed  CAS  Google Scholar 

  19. Rong Y, Geng Z, Lau BHS.GINKGO BILOBA attenuates oxidative stress in macrophages and endothelial cells. Free Radical Biol Med 1996;20: 121–7.

    Article  CAS  Google Scholar 

  20. Crapo JD, McCord JM, Fridovich I. Preparation and assay of superoxide dismutases. Methods Enzymol 1978;53: 382–93.

    Article  PubMed  CAS  Google Scholar 

  21. Chance B, Maehly AC. Assay of catalases and peroxidases. Methods Enzymol 1955;2: 764–75.

    Article  Google Scholar 

  22. Tappel AI. Glutathione peroxidase and hydroperoxides. Methods Enzymol 1978;52: 506–13.

    Article  PubMed  CAS  Google Scholar 

  23. Miwa I, Okuda J. Non-enzymatic reduction of alloxan by reduced nicotinamide nucleotide. Biochem Pharmac 1982;31: 921–5.

    Article  CAS  Google Scholar 

  24. Salah N, Miller NJ, Paganga G, Tijburg L, Bolwell GP, Rice-Evans C. Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants. Arch Biochem Biophys 1995;322: 339–46.

    Article  PubMed  CAS  Google Scholar 

  25. Namiki M. Antioxidants/antimutagens in foods. Crit Rev Food Sci Nutr 1990;29: 273–300.

    Article  PubMed  CAS  Google Scholar 

  26. Dahl MK, Richardson T. Photogeneration of superoxide anion in serum of bovine milk and in model systems containing riboflavin and amino acids. J Dairy Sci 1978;61: 400–7.

    Google Scholar 

  27. Kellogg EW III, Fridovich I. Superoxide, hydrogen peroxide and singlet oxygen in lipid peroxidation by xanthine oxidase system. J Biol Chem 1975;250: 8812–7.

    PubMed  CAS  Google Scholar 

  28. Aurand LW, Boonme NH, Giddings GG. Superoxide and singlet oxygen in milk lipid peroxidation. J Dairy Sci 1977;60: 363–9.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Hygiene, Gifu University School of Medicine, 40 Tsukasa-machi, 500-8076, Gifu, Japan

    Mizue Okada, Ryoichi Inaba & Hirotoshi Iwata

  2. Laboratory on Ageing, Chukyo Junior College, Mizunami

    Yoshinori Okada

Authors
  1. Mizue Okada
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yoshinori Okada
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ryoichi Inaba
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hirotoshi Iwata
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/BF02931795.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Okada, M., Okada, Y., Inaba, R. et al. Scavenging effects of methanolic extracts of broad beans on free-radical species. Environ Health Prev Med 3, 6–11 (1998). https://doi.org/10.1007/BF02931232

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02931232

Key words

Environmental Health and Preventive Medicine

ISSN: 1347-4715

Contact us