- Original article
- Published:
The influence of beryllium on cell survival rates in theIn-vitro culture system, on intracellular DNA synthesis and on SRBC-IgM antibody production responses
Environmental Health and Preventive Medicine volume 2, pages 117–121 (1997)
Abstract
Immunocytotoxicity of beryllium (Be) was evaluated by studying cell viability, intracellular DNA synthesis and SRBC-IgM response in an in-vitro culture system using non-sensitized spleen cells of a C57BL mouse. Be addition showed a suppressive effect on cell viability, an enhancing effect on DNA synthesis and on IgM antibody production. The suppressive effect on cell viability manifested itself markedly as the concentration of Be was increased or the culture time was prolonged. The DNA synthesis-enhancing effect was noted at a relatively low concentration of Be (not more than 10μM). The enhancing effect on the IgM response was related to Be concentration at not more than 20μM. The experimental results mentioned above speculate that the cytotoxicity of Be shows a conflicting pattern of enhancement or suppression according to the concentration used and that immunologically it has a modulating effect or an activating effect on the immunocompetent cells.
References
Yoshida T. Trace metals and immune allergy. Dig Scie Lab 1989 ;12: 14–8.
Kusaka Y. Occupational diseases caused by exposure to sensitizing metals. Jpn J Ind Health 1993 ;35: 75–87.
Yahara I, Edelman GM. Restriction of the mobility of lymphocyte immunoglobulin receptors by concanavalin A. A Proc Nat Acad Sci USA 69 1972: 608–12.
Cunningham AJ. A method of increased sensitivity for detecting single antibody-formming cells. Nature 1965 ;207: 1106–7.
Morita K, Murai Y, Kurita H, Watanabe K, Shima S. Osmotic fragility of humman red cells in vitro treated with soluble beryllium salts. Jpn J Ind Health 1979 ;21: 550–1.
Morita K, Inoue S, Murai Y, Watanabe K, Shima S. Effects of the metals, Be, Fe, Cu and Al, on the mobility of immunoglobulin receptors. Exper 1982 ;38: 1227–8.
Dubos KP, Cochran KW, Mazur M. Inhibition of phosohates by beryllium and antagonism of inhibition by manganese. Sci 1949 ;110: 420–2.
Klemperer FW, Miler JM, Hill CJ. Inhibition of alkaline phosphatase by beryllium. J Biol Chem 1949 ;180: 281–286.
Sakaguchi T, Sakaguchi S, Nakamura I, Kudo Y. The changes of complement activities in sera of mice after subcutaneous administration of beryllium chloride. Jpn J Hyg 1996 ;50: 1077–83.
Nordlind K. Stimulation effect of mercuric chloride and nickel sulfate on DNA synthesis of thymocytes and peripheral lym-phoid cells from newborn guinea pigs. Int Arch Allergy Appl Immunol 1983 ;72: 177–9.
Nordlind K, Henze A. Investigation of ability of the metal allergens cobalt chloride nikel sulfate and potassium dichromate to give a mitogenic response in human thymocytes. Int Arch Allergy Appl Immunol 1984 ;75: 330–2.
Waner GL, Lawerence DA. Stimulation of murine lymphocyte responses by cation. Cell Immunol 1986 ;101: 425–39.
Vaz EM, Vas NM, Levine BB. Presistent formation of reagin in mice injected with low doses of ovalbumin. Immunology 1971 ;21: 11–5.
Okudaira H, Ishizaka K. Reginic antibody formation in the mouse IV. Adoptive anti-hapten IgE antibody response in irradiated recipients of hapten-primed cells and carrier-specific cells. J Immunol 1974 ;113: 563–73.
Shima S, Morita K, Murai Y, Kato Y, Miki T, Fukaya M, Watanabe K, Kurita H, Ito Y, Inoue S, Yamamoto K. Biochemical and electronmicroscopic studies of cyto-agglutinating ability of soluble beryllium salts and its agglutinating cell membrane surface. J Sci Lab 1984 ;60: 149–56.
Author information
Authors and Affiliations
Additional information
An erratum to this article is available at http://dx.doi.org/10.1007/BF02931242.
Rights and permissions
About this article
Cite this article
Yoshida, T., Shima, S., Kurita, H. et al. The influence of beryllium on cell survival rates in theIn-vitro culture system, on intracellular DNA synthesis and on SRBC-IgM antibody production responses. Environ Health Prev Med 2, 117–121 (1997). https://doi.org/10.1007/BF02931976
Issue Date:
DOI: https://doi.org/10.1007/BF02931976